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COMPARISON OF THE ABILITY OF IPSCs INTO GERM CELLS

Thursday, September 17, 2015 — Poster Session II

12:00 p.m. – 1:30 p.m.
FAES Terrace
NICHD
STEMCELL-5

* FARE Award Winner

Authors

  • S Park
  • J Yu
  • AH DeCherney
  • EF Wolff

Abstract

Oocytes were recently derived from induced pluripotent stem cells (iPSCs) via PGC intermediaries in mice, which introduced the possibility of making patient specific gametes in an ethically favorable method. Previous studies have reported that iPSCs exhibit a propensity for differentiation into their original cell lineages, while others did not. In this study, we compared the ability of iPSCs derived from either rhesus ovarian stem cells (OSC) or rhesus fibroblasts (FB) to differentiate into PGCs. We established the first serum-free and defined differentiation protocol for inducing PGC-like cells (PGCLC) from rhesus iPSCs. Efficient reprogramming of both OSC and FB-derived iPSCs (OSC-iPSCs, FB-iPSCs, respectively) was confirmed by the presence of teratoma formation, immunofluorescence staining of pluripotency markers as well as RT-PCR. Using surface marker SSEA1 to enrich for PGCLCs by flow cytometry, we found that FB-iPSCs exhibited higher efficiency in differentiation to PGCLCs compared to OSC-iPSCs (18.33+/-4.03% vs3.07+/-1.34%, respectively P

Category: Stem Cell Biology