Quantitative Nailfold Video Capillaroscopy Parameters Correlate with Dermatomyositis Activity and Damage

Authors

• HK Prakash
• D Song
• D Lichy
• P Rao
• M Jain
• J Shrader
• FW Miller
• A Schiffenbauer
• A Gorbach
• LG Rider

Abstract

Background/Purpose: To assess microvascular structure and function in patients with adult dermatomyositis (DM) and juvenile dermatomyositis (JDM), we have designed and built a mobile, high-resolution Nailfold Video Capillaroscopy (NFVC) installation for clinical use, and correlated the results with both laboratory and clinical measures in patients. Methods: We quantified nailfold capillary features in patients with DM (n=28) and JDM (n=17) with median age of 16.1 yrs (IQR 10.5 – 42.8) and median disease duration of 2.95 yrs (IQR 1.1 - 10.2) (74% females and 59% Caucasian) and healthy control subjects (n=18) with similar demographics. Capillary structural markers, including capillary density, tortuosity, interlimb distance, and functional markers, including local blood velocity in arterial (BVA) and venous (BVV) segments of capillaries, were measured. Left ring finger imaging of the nailfold area was performed with a motorized stereomicroscope (Leica M205C) equipped with a video camera (Hamamatsu ORCA-Flash4.0) at 25x overall magnification, providing 1 µm resolution and up to 100 Hz acquisition rate. Spearman rank correlation coefficients and p-values were calculated to determine correlations between NFVC markers and clinical data. Results: Capillary density (p

Scientific Focus Area: Biomedical Engineering and Biophysics

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