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Nano-proteomic analysis technologies: advancing quantitative proteomic research, biomarker assessment, and molecular profiling

Friday, September 15, 2017 — Poster Session IV

1:00 p.m. – 2:30 p.m.
FAES Terrace
NCI
CANCER-6

Authors

  • J Chen
  • X Luo

Abstract

We offer expertise and provide state-of-the-art immunoassays to facilitate: • Comprehensive and quantitative cell signaling profiling • Nanoscale proteomics analysis • Biomarker & therapeutic target identification and validation • On- and off-target drug activity assessment and pharmacodynamics evaluation Three innovative technologies have been established: 1) Simple WesternTM -- An automated capillary immunoassay system for precise and accurate measurement of proteins and respective post-translational modifications. The system provides highly reproducible and quantitative proteomic analysis with good assay sensitivity and dynamic range. Using nanogram level of protein loading, it enables analysis with extremely small and precious samples. A panel of over two hundred assays, covering key signaling pathways of receptor activation, down-stream signaling transduction, transcriptional regulation, cell cycle control and apoptosis etc., has been developed / established, offering a platform for comprehensive functional proteomic studies in both discovery research and clinical studies. A panel of about 100 assays were also established in PBMC samples. 2) Luminex multiplexing immunoassays -- A multiplex in-solution ELISA analysis system, based on the Luminex xMAP beads technology, to deliver multiplexed assay capabilities with small sample consumption. The system is the most widely cited multiplex immunoassay platform for measurements of cytokine, metabolite, immune response, and serum/plasma biomarker etc. 3) Single-cell western system -- Performs western analysis on 1000-2000 single cells in parallel. The system measures proteins hard-to-detect by conventional single cell analysis platform (e.g. FACS), such as isoforms, phosphorylated proteins, transcription factors, intracellular proteins etc. It offers a tool for single cell signaling study and population heterogeneity dissection at protein level.

Category: Cancer Biology