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NIH Research Festival

September 14 – 16, 2016

Trans-NIH Electron Microscopy Shared Facility

Friday, September 16, 2016 – Poster Session IV
12:00 – 1:30 p.m.

FAES Terrace

NIBIB

STRUCTBIO-5

Authors

  • G Zhang

Abstract

The Trans-NIH Electron Microscopy Facility situated in NIBIB’s Biomedical Engineering and Physical Science Shared Resource provides intramural investigators with tools for characterizing subcellular structure using transmission electron microscopy (TEM), scanning electron microscopy (SEM) and serial block face scanning electron microscopy (SBF-SEM) as well as the associated specimen preparation methods. Available techniques include conventional chemical fixation, embedding and sectioning of cells and tissues for TEM and SBF-SEM, high-pressure freezing and freeze-substitution for optimized specimen preservation, and immunolabeling methods to identify specific proteins based on pre- and post-embedding procedures. The facility provides the capability for determining three-dimensional subcellular structure using automated electron tomography. In addition, investigators have access to methods for determining structure of isolated macromolecular complexes by negative staining, as well as mass mapping based on scanning transmission electron microscopy (STEM) of unstained preparations. The facility includes one of the few SEMs on the NIH campus, providing a capability for determining the surface morphology of cells and biomaterials, and the only one SBF-SEM (Fully automated Zeiss Sigma/Gatan 3View Serial block face SEM) on campus for providing investigators with 3D imaging of tissues (up to 300 microns across and 500 microns in depth) at the nanoscale level. Training is offered for some of the instruments in the facility so that researchers can record their own electron micrographs.

Scientific Focus Area: Structural Biology

This page was last updated on Friday, March 26, 2021

  • General Schedule of Events
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2016 program

Download the 2016 Research Festival Schedule Overview (6 pages)

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Current Research Festival

  • 2025
  • 2024
    • General Schedule of Events
    • 2024 NIH Distinguished Scholars Program
    • Poster Sessions
    • Concurrent Workshops
    • NIH Resource Information Fair
    • National Academy of Science Mini-Symposium
    • Special Wednesday Events
    • Vendor Exhibit Information
    • Green Labs Fair
    • Research Festival Committee
  • 2023
    • General Schedule of Events
    • NIH Early–Career Investigator Lectures
    • Poster Sessions
    • Concurrent Workshops
    • NIH Resource Information Fair
    • Vendor Exhibit Information
    • Research Festival Committee
  • 2021
  • 2019
    • General Schedule of Events
    • Plenary Sessions
    • Poster Session
    • Data Blitz
    • FARE Award Ceremony
    • Special Exhibits on Resources for Intramural Research
    • Technical Sales Association (TSA) Research Festival Exhibit Tent Show
    • Research Festival Committee
  • 2018
    • General Schedule of Events
    • Plenary Sessions
    • Concurrent Symposia Sessions
    • Poster Sessions
    • FARE Award Ceremony
    • Special Exhibits on Resources for Intramural Research
    • Technical Sales Association (TSA) Research Festival Exhibit Tent Show
    • Research Festival Committees
  • 2017
    • General Schedule of Events
    • Plenary Sessions
    • Concurrent Symposia Sessions
    • Poster Sessions
    • FARE Award Ceremony
    • Future Research Leaders
    • Special Exhibits on Resources for Intramural Research
    • Technical Sales Association (TSA) Research Festival Exhibit Tent Show
    • Research Festival Committees
  • 2016
    • General Schedule of Events
    • Plenary Sessions
    • Concurrent Symposia Sessions
    • Poster Sessions
    • Browse by Presenter Name
    • Browse by Presenter IC
    • FARE Award Ceremony
    • Future Research Leaders
    • Special Exhibits on Resources for Intramural Research
    • Technical Sales Association (TSA) Research Festival Exhibit Tent Show
    • Tours of the National Library of Medicine and Clinical Center
    • Research Festival Committees
  • 2015
    • General Schedule of Events
    • Plenary Sessions
    • Concurrent Workshop Sessions
    • Poster Sessions
    • Browse by Presenter Name
    • Browse by Presenter IC
    • FARE Award Ceremony
    • Future Research Leaders
    • Special Exhibits on Resources for Intramural Research
    • TSA Research Festival Exhibit Show
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