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NIH Research Festival

September 14 – 16, 2016

NHLBI Proteomics Core – From proteins to systems biology

Thursday, September 15, 2016 – Poster Session II
12:00 – 1:30 p.m.

FAES Terrace

NHLBI

RSCHSUPP-8

Authors

  • A Aponte
  • Y Chen
  • S Patel
  • G Wang
  • Y Yang
  • M Gucek

Abstract

We are experts in mass spectrometry based proteomics for identification and quantitation of proteins and their posttranslational modifications (PTM). Our workflows for relative protein quantitation are based on label-free, SILAC and TMT approaches. We can also help investigators identify and quantify protein posttranslational modifications, including phosphorylation, nitrosylation, acetylation, ubiquitination, succinylation, etc. We provide training in proper sample preparation and lead the researchers through mass spectrometric analysis to data searching and interpretation. Users have access to a variety of proteomics software platforms (Mascot, Proteome Discoverer, Sequest, Scaffold) for re-searching the data or viewing the results. We offer the following types of analyses: -Protein identification (from in-solution, 1D/2D gel) -Relative protein quantitation (label-free, SILAC, TMT, DIGE) -Peptide/protein fractionation using gels, offline basic reversed LC, IEF or SCX -Protein post-translational modifications – identification and relative quantification (phosphorylation, acetylation, succinylation, malonylation, nitrosylation, ubiquitination, nitration, SUMO, sulfhydration, proline oxidation) -Serum depletion and protein identification/quantification -We work with investigators on custom projects (either targeted proteins or systems biology approaches) Our research is focused on developing new approaches for PTM characterization and absolute protein quantitation (e.g. measuring occupancy of nitrosylation with cys-TMT tags, acetylation occupancy, tissue ubiquitination and absolute quantification of a mitochondrial protein panel).

Scientific Focus Area: Research Support Services

This page was last updated on Friday, March 26, 2021

  • General Schedule of Events
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2016 program

Download the 2016 Research Festival Schedule Overview (6 pages)

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Current Research Festival

  • 2025
  • 2024
    • General Schedule of Events
    • 2024 NIH Distinguished Scholars Program
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    • NIH Resource Information Fair
    • National Academy of Science Mini-Symposium
    • Special Wednesday Events
    • Vendor Exhibit Information
    • Green Labs Fair
    • Research Festival Committee
  • 2023
    • General Schedule of Events
    • NIH Early–Career Investigator Lectures
    • Poster Sessions
    • Concurrent Workshops
    • NIH Resource Information Fair
    • Vendor Exhibit Information
    • Research Festival Committee
  • 2021
  • 2019
    • General Schedule of Events
    • Plenary Sessions
    • Poster Session
    • Data Blitz
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    • Special Exhibits on Resources for Intramural Research
    • Technical Sales Association (TSA) Research Festival Exhibit Tent Show
    • Research Festival Committee
  • 2018
    • General Schedule of Events
    • Plenary Sessions
    • Concurrent Symposia Sessions
    • Poster Sessions
    • FARE Award Ceremony
    • Special Exhibits on Resources for Intramural Research
    • Technical Sales Association (TSA) Research Festival Exhibit Tent Show
    • Research Festival Committees
  • 2017
    • General Schedule of Events
    • Plenary Sessions
    • Concurrent Symposia Sessions
    • Poster Sessions
    • FARE Award Ceremony
    • Future Research Leaders
    • Special Exhibits on Resources for Intramural Research
    • Technical Sales Association (TSA) Research Festival Exhibit Tent Show
    • Research Festival Committees
  • 2016
    • General Schedule of Events
    • Plenary Sessions
    • Concurrent Symposia Sessions
    • Poster Sessions
    • Browse by Presenter Name
    • Browse by Presenter IC
    • FARE Award Ceremony
    • Future Research Leaders
    • Special Exhibits on Resources for Intramural Research
    • Technical Sales Association (TSA) Research Festival Exhibit Tent Show
    • Tours of the National Library of Medicine and Clinical Center
    • Research Festival Committees
  • 2015
    • General Schedule of Events
    • Plenary Sessions
    • Concurrent Workshop Sessions
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