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NIH Research Festival

September 14 – 16, 2016

Nano-Proteomic Analysis Technologies: Advancing Quantitative Proteomic Research, Biomarker Assessment, and Molecular Profiling

Wednesday, September 14, 2016 – Poster Session I
3:00 – 4:30 p.m.

FAES Terrace

NCI

CANCER-14

Authors

  • JQ Chen
  • X Luo

Abstract

The Collaborative Protein Technology Resource (CPTR) is tasked to develop cutting edge protein analysis technologies and provide CCR/NCI/NIH investigators with • comprehensive and quantitative cell signaling event dissection • nanoscale proteomics assessment • biomarker development and therapeutic target identification • clinically applicable assay development and applications To address the challenges of conventional proteomic approaches, such as limited sample size, poor assay reproducibility and unreliable data quantification, a novel automated capillary nano-immunoassay (CNIA) system (Simple WesternTM, ProteinSimple) has been recently deployed and further developed in our core. Over two hundred assays covering key signaling pathways have been established and successfully applied on precise and accurate measurement for signaling molecule activity and quantitative proteomic studies. Robust assay performance using only nanograms of protein makes the CNIA technology appealing for analysis in extremely small and precious samples, such as stem cells, primary cells, fine needle aspirates, and other patient specimens etc. This approach enables the measurement of protein level responses to drug treatment in specimens collected in a non- or minimally- invasive manner. A high-throughput, high-performance in-solution multiplex ELISA system, based on the Luminex xMAP technology, has also been implemented. The system is widely used for cytokine, metabolite, immune response, serum/plasma biomarker analysis in both discovery research and clinical practice. We also actively evaluating, developing, and implementing advanced proteomic analysis technologies. We are implementing a novel single-cell Western system, which performs Western analysis on thousands of single cells in parallel, offers a tool for single cell signaling study and population heterogeneity dissection.

Scientific Focus Area: Cancer Biology

This page was last updated on Friday, March 26, 2021

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2016 program

Download the 2016 Research Festival Schedule Overview (6 pages)

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Current Research Festival

  • 2025
  • 2024
    • General Schedule of Events
    • 2024 NIH Distinguished Scholars Program
    • Poster Sessions
    • Concurrent Workshops
    • NIH Resource Information Fair
    • National Academy of Science Mini-Symposium
    • Special Wednesday Events
    • Vendor Exhibit Information
    • Green Labs Fair
    • Research Festival Committee
  • 2023
    • General Schedule of Events
    • NIH Early–Career Investigator Lectures
    • Poster Sessions
    • Concurrent Workshops
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  • 2021
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    • General Schedule of Events
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    • Technical Sales Association (TSA) Research Festival Exhibit Tent Show
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    • General Schedule of Events
    • Plenary Sessions
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    • FARE Award Ceremony
    • Special Exhibits on Resources for Intramural Research
    • Technical Sales Association (TSA) Research Festival Exhibit Tent Show
    • Research Festival Committees
  • 2017
    • General Schedule of Events
    • Plenary Sessions
    • Concurrent Symposia Sessions
    • Poster Sessions
    • FARE Award Ceremony
    • Future Research Leaders
    • Special Exhibits on Resources for Intramural Research
    • Technical Sales Association (TSA) Research Festival Exhibit Tent Show
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  • 2016
    • General Schedule of Events
    • Plenary Sessions
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    • Browse by Presenter Name
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    • Tours of the National Library of Medicine and Clinical Center
    • Research Festival Committees
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