Multi-modal imaging analyses of A-kinase anchor protein 13 (Akap13) conditional knockout mice

Authors

• KM Baig-Ward
• SA Anderson
• SL Mumford
• VM Diaz
• CW Quaglieri
• X Wu
• T Chu
• BA Klaunberg
• AH DeCherney
• JH Segars

Abstract

We have previously shown that Akap13 (also known as Brx) is essential for cardiac development in a murine model. A complete gene knockout was embryonically lethal due to dilated cardiomyopathy (DCM), pericardial effusion, and heart failure by embryonic day 10.5-11.0. The cardiac pathology included decreased cellularity, thin walled ventricles, and delayed cardiac looping. Staining with proliferation marker Ki-67 showed no increase in apoptosis. These data led us to utilize a Cre-lox conditional deletion strategy where tamoxifen inducible, heart specific knockout mice were generated as an approach to studying Akap13’s role in the adult mouse. MRI, blood pressure, ECHO, and ECG analyses were performed to evaluate the cardiac functional phenotype of these adult Akap13 conditional knockout (cKO) mice. MRIs were obtained using the Bruker Pharmascan 7T. Cardiac output and stroke volume were significantly reduced (p

Scientific Focus Area: Genetics and Genomics

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