Microscopy and digital imaging in the Experimental Immunology Branch

Authors

• MJ Kruhlak

Abstract

The Experimental Immunology Branch (EIB) Microscopy and Digital Imaging Facility is one of the core microscopy facilities within the Center for Cancer Research at the NCI and is operated Dr. Michael Kruhlak. The EIB Microscopy Facility was established to provide investigators access to a range of state-of-the-art microscopy related research tools and techniques including high-resolution fluorescence confocal and multi-photon microscopy. Thereby, the primary mission of the EIB Microscopy Facility is to develop and support the microscopy and digital imaging needs of EIB investigators conducting research in cellular and molecular immunology. In addition, the EIB Microscopy Facility is open to other NCI and NIH scientists interested in developing specialized quantitative microscopy based assays to be applied to studying the cell biology of cancer. Four high quality research grade microscopes are housed in the multi-user facility, with the different instruments providing a wide range of image modes for NCI scientists, from standard immunohistochemistry, through brightfield and wide-field epifluorescence imaging, to highly complex live cell confocal microscopy. Equipment included in the EIB Microscopy Facility includes a Zeiss LSM780 NLO META laser scanning microscope, a Zeiss LSM510 META confocal microscope, and two wide-field epifluorescence microscopes, one configured for automated recognition and imaging of chromosome translocations in metaphase spreads.

Scientific Focus Area: Research Support Services

This page was last updated on Friday, March 26, 2021