Development of a Semi-Automated High-Throughput Metabolic Stability Assay Using an Integrated High-Resolution Accurate Mass Method

Authors

• P Shah
• K Nguyen
• A Wang
• E Kerns
• S Obach
• X Xu

Abstract

Intrinsic clearance (CLint) is an extremely significant parameter for a compound because it influences bioavailability as well as plasma concentrations of the compound. Our goal is to measure CLint for a set of compounds with each major human cytochrome P450 (CYP) isozyme from which in silico prediction tools could be develop to enhance drug discovery, for hit selection, lead optimization, clinical development and projection of dose for human studies. In order to achieve our goal, it is of utmost importance to first develop a robust, sensitive, high-throughput metabolic stability assay. Our assay (384-well format) consisted of two parts; a robotic system for incubation and sample clean up and an integrated LC/Full-scan MS method to calculate the percent remaining of parent compound. The method we developed contains all the necessary information to run the instruments data acquisition as well as the parameters required for processing, data review and reporting as an automated workflow. The assay was evaluated using three long t1/2 compounds propranolol, carbamazepine, antipyrine (>30 mins) and two short t1/2 compounds loperamide, buspirone (

Scientific Focus Area: Research Support Services

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