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Axonal autophagosomes recruit dynein for retrograde transport through fusion with late endosomes

Friday, September 18, 2015 — Poster Session V

2:00 p.m. – 3:30 p.m.
FAES Terrace
NINDS
NEURO-5

* FARE Award Winner

Authors

  • X Cheng
  • B Zhou
  • M Lin
  • Q Cai
  • Z Sheng

Abstract

During autophagy, an essential catabolic cellular pathway, double-membrane autophagosomes are formed and enclose bulk cytosol and/or organelles. Subsequently, autophagosomes either fuse with late endosomes (LEs), generating a hybrid organelle termed amphisome, or fuse with lysosomes for final degradation. Impairment of this pathway is associated with several neurodegenerative disorders. Thus, efficient degradation of autophagic vacuoles (AVs) via lysosomes is critical for cellular homeostasis. This is particularly challenging for polarized cells, like neurons, which have long processes where mature acidic lysosomes are enriched in the soma, but not in axon terminals. Although dynein-driven retrograde transport of AVs has been suggested, a fundamental question remains-how do autophagosomes that are generated at distal axons acquire dynein motors for retrograde transport to the soma. Here, we demonstrate that late endosome (LE)-loaded dynein-snapin complexes drive AV retrograde transport in axons upon fusion of autophagosomes with LEs into amphisomes. Blocking the fusion between autophagosomes and LEs with syntaxin17 knockdown reduced recruitment of dynein motors to AVs, thus immobilizing them in axons. Deficiency in dynein-snapin coupling impaired AV transport, resulting in AV accumulation in neurites and synaptic terminals. Our study provides the first evidence that autophagosomes recruit dynein through fusion with LEs and reveals a new motor-adaptor sharing mechanism by which neurons may remove distal AVs engulfing aggregated proteins and dysfunctional organelles for efficient degradation in the soma.

Category: Neuroscience