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NIH Research Festival

September 23 – 25, 2024

A Rapid Method for Producing Adeno-Associated Viral Vectors Suitable for Transducing Rodent Neurons in vitro and in vivo

Authors

  • DB Howard
  • R Svarcbahs
  • LN Gore
  • BK Harvey
  • CT Richie

Abstract

The use of adeno-associated viral vectors for delivery of genetic information into the mammalian CNS remains popular but producing highly purified vectors for in vivo applications requires a significant investment of resources and time that can impede the development and testing of AAV vectors for experimentation. To address this issue, we have developed a simplified AAV packaging protocol that does not require large capital equipment (ultracentrifugation or chromatography machines) yet still produces virus in quantities that are sufficient for testing AAV prototypes in the rodent CNS. This protocol is serotype agnostic, and has been successful with AAV1, AAV9, AAV-DJ, and rAAV2-retro. Intracranial injection of AAV-EF1a-GFP-KASH into rats demonstrated that our “small scale” AAV preps produce patterns of transgene expression and inflammation that are similar to those produced by the same AAV vector purified by affinity column chromatography. Our protocol allows for multiple vectors to be packaged and processed in parallel, making it ideal for testing multiple variants, constructs, and prototypes simultaneously.

Scientific Focus Area: Neuroscience

This page was last updated on Tuesday, August 6, 2024

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Current Research Festival

  • 2025
    • Posters Session
    • General Schedule of Events
  • 2024
    • General Schedule of Events
    • 2024 NIH Distinguished Scholars Program
    • Poster Sessions
    • Concurrent Workshops
    • NIH Resource Information Fair
    • National Academy of Science Mini-Symposium
    • Special Wednesday Events
    • Vendor Exhibit Information
    • Green Labs Fair
    • Research Festival Committee
  • 2023
    • General Schedule of Events
    • NIH Early–Career Investigator Lectures
    • Poster Sessions
    • Concurrent Workshops
    • NIH Resource Information Fair
    • Vendor Exhibit Information
    • Research Festival Committee
  • 2021
  • 2019
    • General Schedule of Events
    • Plenary Sessions
    • Poster Session
    • Data Blitz
    • FARE Award Ceremony
    • Special Exhibits on Resources for Intramural Research
    • Technical Sales Association (TSA) Research Festival Exhibit Tent Show
    • Research Festival Committee
  • 2018
    • General Schedule of Events
    • Plenary Sessions
    • Concurrent Symposia Sessions
    • Poster Sessions
    • FARE Award Ceremony
    • Special Exhibits on Resources for Intramural Research
    • Technical Sales Association (TSA) Research Festival Exhibit Tent Show
    • Research Festival Committees
  • 2017
    • General Schedule of Events
    • Plenary Sessions
    • Concurrent Symposia Sessions
    • Poster Sessions
    • FARE Award Ceremony
    • Future Research Leaders
    • Special Exhibits on Resources for Intramural Research
    • Technical Sales Association (TSA) Research Festival Exhibit Tent Show
    • Research Festival Committees
  • 2016
    • General Schedule of Events
    • Plenary Sessions
    • Concurrent Symposia Sessions
    • Poster Sessions
    • Browse by Presenter Name
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    • FARE Award Ceremony
    • Future Research Leaders
    • Special Exhibits on Resources for Intramural Research
    • Technical Sales Association (TSA) Research Festival Exhibit Tent Show
    • Tours of the National Library of Medicine and Clinical Center
    • Research Festival Committees
  • 2015
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