Qualitative Tissue Analysis Using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight (MALDI-TOF)

Authors

  • Z Saleem
  • L Scarpelli
  • S Burks
  • J Frank
  • D Agrohia
  • H Kalish

Abstract

Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry is a versatile analytical technique capable of targeting a wide range of biomolecules. MALDI-TOF imaging mass spectrometry facilitates the label-free acquisition of qualitative data in a two-dimensional format, enhancing our understanding of molecular distributions. Tissue slides undergo a series of washes and are coated with specialized matrices to optimize lipid, peptide, or protein expression. This preparation is critical for accurate analysis. Statistical analysis produces heat maps and identifies specific regions within tissues, providing detailed spatial molecular information. MALDI-TOF imaging mass spectrometry offers valuable insights into the spatial molecular characteristics of various tissue types. Its unique capabilities make it an indispensable tool in biomarker detection and discovery, ensuring reliable and reproducible results.

Scientific Focus Area: Immunology

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