NCI/CCR/LRBGE Optical Microscopy Core (Building 41)

Authors

• TS Karpova
• DA Ball
• M Fazel

Abstract

Optical Microscopy Core (NIH/CCR/LRBGE) specializes on biophysical quantification of the live intracellular molecular dynamics by Single Molecule Tracking (SMT) and mapping of the molecules within the cellular organelles (Molecular Mapping by Super-Resolution). For SMT, we offer custom-built instrumentation, based on HILO technology, and custom-designed quantification software. For the molecular maps, we offer Single Molecule Localization Methods (SMLM) such as STORM/DNA-PAINT technology (Elyra from Carl Zeiss Inc) and MINFLUX technology (MINFLUX from Abberior), improved by custom analysis software. These methods rely on sophisticated instrumentation and analysis of the data. Our expertise comes not only from helping users, but also from our involvement in the development of these technologies.
In addition, we provide support in conventional microscopy techniques, from the multi-channel fluorescence imaging of fixed cells and tissue sections, to the quantitative FRAP analysis and FRET by acceptor photobleaching, to the time lapse imaging of cells in 2D or 3D, and computational removal of out-of-focus light (deconvolution). NCI Optical Microscopy Core is a shared resource open to everyone within the NCI, and we also welcome collaborations with investigators from other NIH institutes or beyond. We provide expert guidance and troubleshooting in the design of experiments, their execution, interpretation and publication.

Scientific Focus Area: Research Support Services

This page was last updated on Tuesday, August 6, 2024