Automated, High Throughput mRNA-LNP Formation Using Adaptable Microfluidics

Authors

• ND Jones
• ML Figur
• P Rezvan Sangsari
• JM Groff
• JM Burd
• NY Morgan
• DB Gowetski
• EC Ihms

Abstract

Messenger RNA-loaded lipid nanoparticle (mRNA-LNP) vaccines have proven to be a valuable technology in the public health field due to their efficacy and shorter time to market than traditional protein-based vaccines. High-throughput automation of LNP vaccine assembly process and formulation development can be employed to improve consistency and maximize the use of limited early-stage materials while reducing risk of sample waste. In this work we fabricate and characterize a custom microfluidics-based mRNA-LNP formation device suitable for integration with the Tecan Fluent automated liquid handler. We evaluate the performance of this device in several small-scale development experiments and by performing extensive comparison studies against Cytiva's Ignite/Blaze line of LNP production instruments. By using the same mixer geometry as these commercial instruments, our microscale device can ensure efficient and rapid screening that is immediately suitable for scale-up operations.

Scientific Focus Area: Biomedical Engineering and Biophysics

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