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NIH Research Festival

September 12 – 14, 2018

Proteomic Analysis Technologies: Advancing Quantitative Proteomic Research, Biomarker Assessment, and Molecular Profiling

Wednesday, September 12, 2018 – Poster Session II
3:30 – 5:00 p.m.

FAES Terrace

NCI

CANCER-7

Authors

  • J CHEN
  • K NOEMI
  • X LUO

Abstract

We offer expertise and provide state-of-the-art immunoassays to facilitate: • Comprehensive and quantitative cell signaling profiling • Single-cell protein analysis • Biomarker & therapeutic target identification and validation • On‐ and off‐target drug activity assessment and pharmacodynamics evaluation Technology platforms: 1) Simple WesternTM -- An automated capillary immunoassay system for precise and accurate measurement of proteins and respective post-translational modifications in a format that is applicable to very small sample sizes. The technology provides high-performance assays for comprehensive and quantitative signaling molecule profiling at the protein level and facilitates transferring those assays from discovery research into preclinical/clinical practice. A panel of about three hundred assays, covering key signaling pathways, has been established. 2) Luminex xMAP assays -- A multiplex in-solution ELISA analysis system with small sample consumption. The system provides highly quantitative assays for measurement of multiple cytokines, metabolites, and serum/plasma biomarkers in the same sample. 3) CODEX system -- an immunofluorescence imaging platform for highly multiplexed (30-50 plex) IHC-analysis with single-cell resolution and retention of spatial context. It allows comprehensive in-situ protein profiling with multi-parametric readouts from a single tissue section. It offers a strategy for deep phenotyping of cellular microenvironment and studying its impact on cell fate and function. 4) Single-cell western -- Performs western analysis on 1000-2000 single cells in parallel. Enables detection of proteins hard-to-detect by conventional single-cell analysis platform (e.g. FACS), such as isoforms, phosphorylated proteins, transcription factors, intracellular proteins etc. It offers a tool for studying single-cell signaling and dissection of population heterogeneity at protein level.

Scientific Focus Area: Cancer Biology

This page was last updated on Friday, March 26, 2021

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2018 program

Download the 2018 Research Festival Schedule Overview (6 pages)

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Current Research Festival

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  • 2024
    • General Schedule of Events
    • 2024 NIH Distinguished Scholars Program
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    • National Academy of Science Mini-Symposium
    • Special Wednesday Events
    • Vendor Exhibit Information
    • Green Labs Fair
    • Research Festival Committee
  • 2023
    • General Schedule of Events
    • NIH Early–Career Investigator Lectures
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  • 2021
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    • General Schedule of Events
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    • Data Blitz
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    • Special Exhibits on Resources for Intramural Research
    • Technical Sales Association (TSA) Research Festival Exhibit Tent Show
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  • 2018
    • General Schedule of Events
    • Plenary Sessions
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    • FARE Award Ceremony
    • Special Exhibits on Resources for Intramural Research
    • Technical Sales Association (TSA) Research Festival Exhibit Tent Show
    • Research Festival Committees
  • 2017
    • General Schedule of Events
    • Plenary Sessions
    • Concurrent Symposia Sessions
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    • FARE Award Ceremony
    • Future Research Leaders
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    • Research Festival Committees
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