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Proteomic Analysis Technologies: Advancing Quantitative Proteomic Research, Biomarker Assessment, and Molecular Profiling

Wednesday, September 12, 2018 — Poster Session II

3:30 p.m. – 5:00 p.m.
FAES Terrace
NCI
CANCER-7

Authors

  • J CHEN
  • K NOEMI
  • X LUO

Abstract

We offer expertise and provide state-of-the-art immunoassays to facilitate: • Comprehensive and quantitative cell signaling profiling • Single-cell protein analysis • Biomarker & therapeutic target identification and validation • On‐ and off‐target drug activity assessment and pharmacodynamics evaluation Technology platforms: 1) Simple WesternTM -- An automated capillary immunoassay system for precise and accurate measurement of proteins and respective post-translational modifications in a format that is applicable to very small sample sizes. The technology provides high-performance assays for comprehensive and quantitative signaling molecule profiling at the protein level and facilitates transferring those assays from discovery research into preclinical/clinical practice. A panel of about three hundred assays, covering key signaling pathways, has been established. 2) Luminex xMAP assays -- A multiplex in-solution ELISA analysis system with small sample consumption. The system provides highly quantitative assays for measurement of multiple cytokines, metabolites, and serum/plasma biomarkers in the same sample. 3) CODEX system -- an immunofluorescence imaging platform for highly multiplexed (30-50 plex) IHC-analysis with single-cell resolution and retention of spatial context. It allows comprehensive in-situ protein profiling with multi-parametric readouts from a single tissue section. It offers a strategy for deep phenotyping of cellular microenvironment and studying its impact on cell fate and function. 4) Single-cell western -- Performs western analysis on 1000-2000 single cells in parallel. Enables detection of proteins hard-to-detect by conventional single-cell analysis platform (e.g. FACS), such as isoforms, phosphorylated proteins, transcription factors, intracellular proteins etc. It offers a tool for studying single-cell signaling and dissection of population heterogeneity at protein level.

Category: Cancer Biology