Low-cost HPV testing and the prevalence of cervical infection in asymptomatic populations in Guatemala

Authors

• MR Maria
• H Lou
• E Gharzouzi
• SP Guerra
• JF Domgue
• J Sawitzke
• G Villagran
• L Garland
• J Boland
• S Wagner
• J Martinez
• H Rosas
• J Troxler
• H McMillen
• B Kessing
• E Alvirez
• M Castillo
• M Schiffman
• R Orozco
• M Dean

Abstract

Background: A low cost and accurate method for detecting high-risk (HR) HPV is important to permit HPV testing for cervical cancer prevention. We validated a low-cost commercially available HPV method (H13, Hybribio, Hong Kong) and determined the distribution of HPV infections in over 1717 cancer-free women in Guatemala. Methods: H13 results were compared with two more established HPV tests: (Xpert™ (Cepheid) and SPF10-LIPA25™ (DDL)) in 40 mainly known positive specimens. HR-HPV was detected in cervical samples from 1717 cancer-free women receiving Pap smears using the Hybribio™ real-time PCR assay of 13 HR types. Selected HPV positive samples were sequenced to determine viral type. Results: The Hybribio H13 Assay showed 93% identical results with Xpert, and 89% with SPF10-LIPA25. A total of 13% (226/1717) of women tested HPV+. The highest prevalence was found in younger women (

Scientific Focus Area: Microbiology and Infectious Diseases

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