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Low-cost HPV testing and the prevalence of cervical infection in asymptomatic populations in Guatemala

Wednesday, September 13, 2017 — Poster Session II

3:30 p.m. – 5:00 p.m.
FAES Terrace


  • MR Maria
  • H Lou
  • E Gharzouzi
  • SP Guerra
  • JF Domgue
  • J Sawitzke
  • G Villagran
  • L Garland
  • J Boland
  • S Wagner
  • J Martinez
  • H Rosas
  • J Troxler
  • H McMillen
  • B Kessing
  • E Alvirez
  • M Castillo
  • M Schiffman
  • R Orozco
  • M Dean


Background: A low cost and accurate method for detecting high-risk (HR) HPV is important to permit HPV testing for cervical cancer prevention. We validated a low-cost commercially available HPV method (H13, Hybribio, Hong Kong) and determined the distribution of HPV infections in over 1717 cancer-free women in Guatemala. Methods: H13 results were compared with two more established HPV tests: (Xpert™ (Cepheid) and SPF10-LIPA25™ (DDL)) in 40 mainly known positive specimens. HR-HPV was detected in cervical samples from 1717 cancer-free women receiving Pap smears using the Hybribio™ real-time PCR assay of 13 HR types. Selected HPV positive samples were sequenced to determine viral type. Results: The Hybribio H13 Assay showed 93% identical results with Xpert, and 89% with SPF10-LIPA25. A total of 13% (226/1717) of women tested HPV+. The highest prevalence was found in younger women (

Category: Microbiology and Infectious Diseases