High-Throughput 3D Imaging of the Human Genome

Authors

• Sigal Shachar
• Gianluca Pegoraro
• Ziad Jowhar
• Armin Raznahan
• Tom Misteli

Abstract

The genome is one of the major physical entities in every cell and is complexly organized in space and time. High-throughput imaging approaches are emerging as powerful tools to elucidate the cell biological properties of genomes and to link genome architecture to function at a single-cell level. Deep Imaging methods are based on the development of high-capacity, high-precision automated microscopes which allow acquisition of large imaging datasets and the implementation of computational image analysis and data mining methods to quantitatively capture morphological phenotypes. Deep Imaging enables new experimental strategies for the study of the genome including visualization and analysis of rare events such as chromosome breaks and translocations, use of large-scale imaging-based screens to probe molecular mechanisms of genome organization and function in an unbiased fashion, and they allow mapping of the genome in 3D space. These approaches are powerful tools to probe the cell biology of genomes and provide novel insights into genome architecture and function.

This page was last updated on Friday, March 26, 2021