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NIH Research Festival

September 13 – 15, 2017

High Resolution Flow Cytometry Analysis of Activated T Cells

Friday, September 15, 2017 – Poster Session IV
1:00 – 2:30 p.m.

FAES Terrace

NEI

IMMUNO-5

Authors

  • JA Minehart
  • RS Villasmil
  • B Xu

Abstract

Examination of T cell function is one of the most frequently used output readings in immunological research laboratories around the world. Here, we review multiple high resolution flow cytometry techniques for the study of T cell activation. These techniques include immunophenotyping, viability analysis and proliferation monitoring. Conventional cytometers often lack the resolving power to differentiate the activated and unactivated T cell populations by light scatter detection and quantitative surface antigen measurement. Using a novel cytometer, which measures light scatter and surface antigen parameters at high resolution, we discriminated proliferating and non-proliferating populations with more accurate differentiation than commonly reported. High resolution in flow cytometric data enables better distinction between T cell activation states.

Scientific Focus Area: Immunology

This page was last updated on Friday, March 26, 2021

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2017 program

Download the 2017 Research Festival Schedule Overview (6 pages)

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Current Research Festival

  • 2025
  • 2024
    • General Schedule of Events
    • 2024 NIH Distinguished Scholars Program
    • Poster Sessions
    • Concurrent Workshops
    • NIH Resource Information Fair
    • National Academy of Science Mini-Symposium
    • Special Wednesday Events
    • Vendor Exhibit Information
    • Green Labs Fair
    • Research Festival Committee
  • 2023
    • General Schedule of Events
    • NIH Early–Career Investigator Lectures
    • Poster Sessions
    • Concurrent Workshops
    • NIH Resource Information Fair
    • Vendor Exhibit Information
    • Research Festival Committee
  • 2021
  • 2019
    • General Schedule of Events
    • Plenary Sessions
    • Poster Session
    • Data Blitz
    • FARE Award Ceremony
    • Special Exhibits on Resources for Intramural Research
    • Technical Sales Association (TSA) Research Festival Exhibit Tent Show
    • Research Festival Committee
  • 2018
    • General Schedule of Events
    • Plenary Sessions
    • Concurrent Symposia Sessions
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    • FARE Award Ceremony
    • Special Exhibits on Resources for Intramural Research
    • Technical Sales Association (TSA) Research Festival Exhibit Tent Show
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  • 2017
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    • Future Research Leaders
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