Biomarker Detection Using MALDI Imaging
Friday, September 15, 2017 — Poster Session IV
- M Davisson
- G Fabozzi
- C Petrovas
- H Kalish
Determining the location and relative concentrations of specific proteins within tissue samples is important to the biomedical community. Certain proteins, found in high concentrations, can be indicative of infected or diseased tissues. Confocal microscopy has been used to image target proteins in tissues using fluorescent dye and antibodies, but this technique is limited by advance knowledge of proteins in the sample. Matrix assisted laser desorption ionization (MALDI) mass spectrometry imaging is a new technique used to image proteins, peptides and lipids found in tissue samples. By using MALDI mass spectrometry in tandem with confocal microscopy, our goal is to identify new biomarkers of interest and to confirm the presence of known biomarkers in infected tissues.