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Small molecule inhibitors of Zika virus replication identified from a drug repurposing screen

Friday, September 16, 2016 — Poster Session IV

12:00 p.m. – 1:30 p.m.
FAES Terrace
NCATS
VIROL-2

Authors

  • Miao Xu
  • Jenni Kouznetsova
  • Ruili Huang
  • Wenwe Huang
  • Misha Itkin
  • Paul Shinn
  • Samue Michael
  • Anton Simeonov
  • Mengh Xia
  • Wei Zheng

Abstract

Zika virus infects human neural progenitor cells and astrocytes resulting in increased caspase-3 activity and cell death. We have developed a high throughput caspase-3 activity assay and a cell viability assay in 1536-well plates using Zika virus infected human cells. A screening of FDA approved drugs and clinical trial stage drugs led to identification of two groups of compounds including a protease inhibitor (IC50 = 0.13 µM) and protect cell from death (IC50 = 1.06 µM) caused by ZIKV infection. We also found two groups of compounds that inhibited ZIKV replication in human cells in dosage-dependent manner. Our results has validated this compound screening strategy and provided leads for drug development to treat infections with Zika virus infections.

Category: Virology