NIH Research Festival
Zika virus infects human neural progenitor cells and astrocytes resulting in increased caspase-3 activity and cell death. We have developed a high throughput caspase-3 activity assay and a cell viability assay in 1536-well plates using Zika virus infected human cells. A screening of FDA approved drugs and clinical trial stage drugs led to identification of two groups of compounds including a protease inhibitor (IC50 = 0.13 µM) and protect cell from death (IC50 = 1.06 µM) caused by ZIKV infection. We also found two groups of compounds that inhibited ZIKV replication in human cells in dosage-dependent manner. Our results has validated this compound screening strategy and provided leads for drug development to treat infections with Zika virus infections.
Scientific Focus Area: Virology
This page was last updated on Friday, March 26, 2021