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Human Artificial Chromosomes (HACs) Vectors for Human Gene Delivery and Drug Screening

Friday, September 18, 2015 — Poster Session IV

12:00 p.m. – 1:30 p.m.
FAES Terrace
NCI
GEN-26

Authors

  • JH Kim
  • HS Lee
  • NC Lee
  • N Kouprina
  • V Larionov

Abstract

Human artificial chromosomes (HACs) based vectors have several advantages over viruses as gene delivery vectors and the ability to carry large gene inserts. HACs avoid the limited cloning capacity, lack of copy number control, and insertional mutagenesis caused by integration into host chromosomes that plague viral vectors. HAC vectors also have a great potential for screening of anticancer drug. HACs allow therapeutic transgenes to be expressed in target cells under conditions that recapitulate the physiological regulation of endogenous loci. Here we summarize our recent work on gene transfer into human cells using the HACs for complementation studies as well as the use of HACs for screening of new drugs affecting chromosome instability.

Category: Genetics and Genomics