NIH Research Festival
Dengue virus is a member of the Flavivirus genus of viruses and can result in severe febrile illness. Here we show for the first time that the uncharacterized protein FLJ11286, which we refer to as IRAV, is induced by dengue in an interferon dependent manner and displays antiviral activity against dengue virus. IRAV associates with dengue and host proteins, and interacts with MOV10. IRAV is also associated with cytoplasmic processing bodies (p-bodies). We demonstrate that IRAV is an RNA binding protein and interacts with DENV RNA. Furthermore, we show that overexpression of IRAV results in a diminished stability of dengue virus mRNA, suggesting a role for IRAV in mediating the stability of viral RNA. These data serve to identify a novel interferon stimulated gene with antiviral activity against certain viruses as well as to propose a mechanism of activity involving interactions with, and destabilization of viral mRNA.
Scientific Focus Area: Institute, Center, and Scientific Directors
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