Advances in molecular microscopy

Tuesday, September 23, 2014 — Concurrent Symposia Session III
2:00 p.m. – 4:00 p.m.	Classrooms 6 and 7

Chair

• Sriram Subramaniam, NCI

Program

Understanding the dynamic architecture of the molecular machines at the heart of cell function is a problem of broad and fundamental biological interest at the interface between structural and cell biology. The vast majority of these macromolecular complexes are generally too large and/or too heterogeneous to be investigated by established methods such as X-ray crystallography, and the level of detail afforded by conventional light and electron microscopy is often not adequate to decipher detailed molecular mechanisms. Recent technological breakthroughs in 3D electron microscopy and in super-resolution light microscopy have opened up new vistas for structural imaging at the molecular and cellular level and provide unprecedented opportunities for describing the structure and dynamics of a large variety of biologically and medically relevant protein complexes. The talks in this symposium will cover some of the exciting areas where progress is being made on both technical and biological fronts.

Imaging the molecular architecture of the plasma membrane with correlative 3D super resolution light and electron microscopy
Justin Taraska, NHLBI

Nanoscale 3D Imaging of Cells and Tissues with Scanned Electron Probes
Richard Leapman, NIBIB

Exploring Diversity in Myosin II Filaments Using Structured-Illumination Microscopy; FARE award winner
Jordan Beach, NHLBI

Cytoskeletal structural plasticity in force generation and mechanosensation
Greg Alushin, NHLBI

Superferritin - visualizing the encapsulin nanocompartment of Myxococcus xanthus
Alasdair Steven, NIAMS

Cryo-electron microscopy comes of age: Protein structures at atomic resolution without crystallography
Sriram Subramaniam, NCI

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