Increasing throughput by automation of manual ELISA on robotic liquid handling platform

Tuesday, September 23, 2014 — Poster Session III
12:00 p.m. – 2:00 p.m.	FAES Academic Center	NCI	CLIN-6

Authors

• J Govindharajulu
• G Barger
• A. K. Srivastava

Abstract

Implementation of pharmacodynamic biomarkers to support DCTD’s anti-cancer drug development program requires us to analyze large number of specimens generated from preclinical translational studies, typically by in-house developed ELISA. Manual ELISA is labor intensive, multistep and mostly time consuming process. Transferring manual ELISA to automated platform can be effective in reducing the labor cost and, at the same time increasing throughput, assay precision and safety. We describe automation of ELISA on a Tecan EVO150 platform equipped with a liquid handling pipettor arm and a robotic manipulator. We integrated EVO150 with Tecan M1000 microplate reader, orbital shakers, heat exchange block, Tecan Hydrospeed plate washer, and a Luminex 200 reader. Programming scripts were developed in standard Freedom EVOware software. Automation was achieved for simultaneous measurement of three manual ELISA assays: total MET, pY1234/1235-MET, and pY1356-MET. The sample throughput was increased at least 3 folds with respect to the manual ELISA, which can be further increased by running workstation in two shifts. Detailed configuration and automation script will be discussed to support rapid adoption of manual ELISA. The workstation is now extended to perform Luminex multiplex immunoassays. Funded by NCI Contract No HHSN261200800001E.

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