Hyperpolarized 13C-pyruvate magnetic resonance imaging of prostate cancer tumor xenografts

Wednesday, September 24, 2014 — Poster Session IV
10:00 a.m. –12:00 p.m.	FAES Academic Center	NCI	CANCER-20

* FARE Award Winner

Authors

• B.T. Scroggins
• M. Matsuo
• A.O. White
• S. Matsumoto
• J.B. Mitchell
• M.C. Krishna
• D.E. Citrin

Abstract

Purpose: Tumor metabolism has been reported to impact solid tumor sensitivities to radiation therapy. Highly glycolytic tumors may be less sensitive to radiation. Non-invasive magnetic resonance imaging (MRI) of hyperpolarized 13C-labeled pyruvate and its metabolite lactate may be useful to profile the metabolism of solid tumors. Methods: Metabolic profiling of cell lines was conducted in vitro using extracellular flux analyzer, mass spectrometry, western blotting, and biochemical assays. In vivo analysis of PC3 and DU145 tumors xenografts in athymic nude mice were analyzed by hyperpolarized 13C MRI. Immunohistochemical and metabolomics analysis was conducted on tumors xenografts to measure metabolic markers. Results: In vitro metabolic analysis showed that PC3 and DU145 cells have similar metabolic profiles. However, in vivo analysis of PC3 and DU145 tumor xenografts by hyperpolarized MRI revealed that lactate production was significantly higher in DU145 tumors than in PC3 tumors. Conclusions: In vitro analysis of PC3 and DU145 cells suggests that there is little difference in many of the markers of glycolysis between these cell lines. However, hyperpolarized 13C MRI analysis indicates that DU145 and PC3 tumors have substantially different glycolytic states in vivo suggesting that tumor physiology may alter metabolism.

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