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Notes from the RNA-Seq Revolution: Deep Sequencing Transcribed RNA in Health and Disease

Tuesday, October 25, 2011 — Concurrent Symposia Session III

2:00 p.m. – 4:00 p.m.

Ruth L. Kirschstein Auditorium


  • Francis McMahon, NIMH
  • Mark Cookson, NIA


RNA-Seq is a revolutionary new technique that leverages high-throughput sequencing technologies to provide estimates of transcript abundance at a precision not previously realized in hybridization-based microarray profiling. RNA-Seq also enables the detection of novel, low-abundance transcripts, allele-specific expression, alternative splicing, and posttranscriptional modifications, such as RNA editing. This technique thus provides a fundamental context in which the impact of genetic variation on gene expression can be evaluated in various cells and tissues. This Symposium will give an overview of ongoing RNA-Seq work in several institutes. Tissue types under study range from brain to platelets, diseases from bipolar disorder to atherosclerosis. Methodological issues will also be addressed, including determining alternative splicing, elucidating novel transcripts, and bioinformatic annotation.

Exploring the Transcriptional Complexity of the Brain
Mark Cookson, NIA

RNA-seq Analysis of Human and Mouse Platelet Transcriptomes
Andrew Oler, NIAID

RNA-Seq in Brain Tissue from Patients with Bipolar Disorder
Francis McMahon, NIMH

Transcriptome Profiling of Cardiovascular Disease by Massively Parallel Short-Read DNA Sequencing *FARE Award Winner
Shurjo Sen, NHGRI

Bioinformatic Challenges of Working with RNA-Seq Data
Nirmala Akula, NIMH

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