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Quantitative proteomics analysis for DHA-enhanced proliferation of neural stem cells

Tuesday, October 25, 2011 — Poster Session II

Noon – 2:00 p.m.

Natcher Conference Center

NIAAA

STEMCELL-3

Authors

  • B Huang
  • M Katakura
  • H Kim

Abstract

Docosahexanoic acid (DHA), an n-3 polyunsaturated fatty acid highly enriched in neuronal membranes, plays important roles in the development of the central nervous system. Neurogenesis of the mammalian brain is originated from neural stem cells (NSCs), mostly during the development with the exception of some brain regions such as dentate gyrus of hippocampus and olfactory bulb. We found that DHA promotes the proliferation of NSCs, in comparison to monounsaturated fatty acid such as oleic acid. To understand the underlying molecular mechanisms, we investigated the differential expression profile of proteins in NSCs. NSCs from E14.5 rats were cultured as neurospheres in N2 medium containing basic fibroblast growth factor (bFGF). Dissociated NSCs were cultured with 1 ┬ÁM DHA or OA in the medium containing bFGF for 4 days. Proteins were extracted, applied to SDS-PAGE, analyzed by nano-LC-ESI-MS/MS, and quantified by label free protein quantitation software. Our results indicated that 200 among 9000 proteins identified in the NSCs were either up- or down-regulated in DHA treated samples. Protein network and pathway analysis indicated that many of these proteins were involved in regulating cell proliferation and biosynthetic process, providing a mechanistic base for the biological function of DHA in proliferation of neural stem cells.

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