Noninvasive characterization of mouse and human glioblastoma multiforme

Wednesday, October 26, 2011 — Poster Session III
10:00 a.m. – Noon	Natcher Conference Center	NCI	IMAG-13

Authors

• SA Jansen
• Y Song
• TN O'Sulilvan
• L Ileva
• B Hughes
• P Mulhern
• E Siegal
• J Chen
• T Van Dyke

Abstract

Noninvasive imaging plays several important roles in the clinical management of patients with glioblastoma multiforme (GBM), from initial diagnosis to monitoring response to therapy. A primary goal of mouse modeling of human cancer is to recapitulate salient features of human disease, not only at a genetic or molecular level but also from an imaging perspective as well. The goal of this study was to perform an imaging-based classification of mouse models of GBM, and to use caBIG® technologies as a framework for performing comparisons with human disease. Primary cell cultures derived from GBM tumors arising in inducible GEMs with clinically relevant genetic alterations (Rb inactivation, Ras activation, PTEN loss and EGFR loss) were injected intra-cranially into syngeneic mice. Standardized methods and terminology utilized for characterizing human GBM were applied to the murine data by using caBIG® technology. Using these tools we found a remarkable similarity between murine and human GBM imaging features. Furthermore, image-based quantification of blood brain barrier permeability showed interesting variability among the genotypes studied. This study demonstrates that application of standardized methods and analysis, facilitated by the caBIG® framework, can play an important role in the validation and characterization of preclinical mouse models of GBM.

back to top