Thousands of knockout mouse strains are available from the KOMP Repository www.komp.org/

Tuesday, October 25, 2011 — Poster Session II
Noon – 2:00 p.m.	Natcher Conference Center	NCRR	GEN/GENOM-14

Authors

• R O'Neill
• P de Jong
• KC Lloyd

Abstract

More than 6,500 different mouse ES cell lines carrying specific gene knockouts can be purchased for less than $650 each by NIH/CBER research investigators (http://komp.org/fees.php). More than 9,000 KO vectors useful for targeting recombination in ES cells, and more than 400 KO lines of mice or sperm are available. Approximately 300 mouse strains have phenotypic data available http://www.kompphenotype.org). The overall objective of NIH's KOMP is to identify the function of 8,500 protein coding genes. Many of the knocked-out genes may be embryonic lethals when homozygous. Several vector constructs (diagrammed at http://komp.org/alleles.php#deletion-csd) are used to make KOs, and conditional vectors allow tissue-specific or cell-type-specific perturbations. Cre-driver mice are available from http://www.creportal.org/ and http://www.credrivermice.org/. Nineteen NIH Institutes and Centers funded KOMP in 2006-2011, with the repository physically located at the University of California, Davis and the Children's Oakland Research Institute; with major partners at the Sanger Institute in the U.K.; Velocigene at Regeneron, Inc.; and the Jackson Laboratory. Twenty ICs and Centers, including the Common Fund through DPCPSI, are expected to fund KOMP2 (http://commonfund.nih.gov/KOMP2/) in 2011-2016. KOMP2 will convert the IKMC knockout embryonic stem (ES) cell libraries into 2,500 lines of mice that have comprehensive phenotypic data.

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