Protein expression and survival in esophageal squamous cell carcinoma (ESCC) cases from China

Tuesday, October 25, 2011 — Poster Session II
Noon – 2:00 p.m.	Natcher Conference Center	NCI	EPID-4

Authors

• V Burton
• C Conway
• N Hu
• S Hewitt
• P Taylor

Abstract

Esophageal squamous cell carcinoma (ESCC) is the 6th most common cause of cancer death worldwide, and over half of all ESCC deaths occur in China. Understanding molecular changes in ESCC will enable the identification of potential targets for early detection and therapy. Previous RNA expression array profiling in 53 ESCC cases from Shanxi Province, a high risk ESCC region in China, identified 159 dysregulated genes, including 32 whose tumor expression levels were associated with survival. To extend and confirm these initial RNA-based findings, the present study was undertaken to evaluate the relation of protein expression of these genes to survival in a large group of new ESCC cases. Formalin fixed, paraffin embedded tumor and matched adjacent normal tissue from 300 newly-diagnosed ESCCs from Shanxi Province who had undergone surgical resection of their tumor were used to construct a tissue microarray (TMA). Cases or their families were subsequently re-contacted to ascertain vital status. Immunohistochemistry (IHC) with image analysis (IA) will be performed on target genes, and tumor expression and the ratio of tumor:normal expression will be related to survival time.

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