NHLBI Electron Microscopy Core Facility

Thursday, October 27, 2011 — Core Poster Session
10:00 a.m. – Noon	South Lobby of Building 10	NHLBI	CORE-6

Authors

• P Connelly
• S Esfahani
• M Daniels

Abstract

The NHLBI Electron Microscopy Core Facility offers consultation, technical services, training and use of the facilities for electron microscopy to all investigators in the NHLBI/DIR. We have collaborated with approximately 50% of all NHLBI investigators since the Core was formed in 2006, and in 2010 we worked with the research groups of 18 principle investigators in support of 22 projects. The facility also offers limited training and use of its electron microscopes as well as very limited collaboration on projects of mutual interest with scientists outside of the NHLBI. The facility is equipped with two 120kV transmission electron microscopes and one scanning electron microscope as well as the preparative equipment for most biological EM applications. Our technical capabilities include: 1) transmission electron microscopy and scanning electron microscopy of tissues, cells and other biological or material samples; 2) immunocytochemistry at the electron microscopic level including immuno-gold labeling; 3) negative staining and rotary shadowing of macromolecular preparations (nucleic acids or proteins) as well as other small structures such as viruses, protein polymers and liposomes. We offer training in the use of both the transmission electron microscope and the scanning electron microscope as well as in the interpretation of biological ultrastructure.

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