Biophysics Resource in the Structural Biophysics Laboratory

Thursday, October 27, 2011 — Core Poster Session
10:00 a.m. – Noon	South Lobby of Building 10	NCI	CORE-20

Authors

• S Tarasov
• M Dyba
• RA Byrd

Abstract

Biophysics Resource is a unique division of the Structural Biophysics Laboratory where NCI scientists can get access to advanced biophysical technologies such as: 1. Isothermal titration calorimetry for thermodynamic characterization of biomacromolecular interactions; 2. Dynamic and static light scattering to determine macromolecular mass, size, and degree of aggregation of biomacromolecules; 3. Circular dichroism (CD) spectroscopy to study optical activity and conformation of biomacromolecules; 4. Liquid chromatography with mass spectrometry detection (LC-MS and LC-MS-MS) for macromolecular mass characterization (including high-resolution option) and for study of mechanisms of biochemical reactions; 5. Steady-state fluorescence spectroscopy to study the structure and environmental sensitivity of biomacromolecules and their interactions; 6. Differential scanning calorimetry for determining thermal stability and transitions in biomacromolecular systems. The Resource operates as an open, shared-use facility. Users get necessary training and perform their own experiments on practically all BR instruments. BR staff members provide technical and collaborative help with the experimental design, data acquisition, analysis, and interpretation up to extent required.

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