NHLBI Proteomics Core Facility

Thursday, October 27, 2011 — Core Poster Session
10:00 a.m. – Noon	South Lobby of Building 10	NHLBI	CORE-2

Authors

• A Aponte
• Y Chen
• S Patel
• S Swatkoski
• G Wang
• M Gucek

Abstract

The mission of the NHLBI Proteomics Core Facility is to provide investigators at the NHLBI access to mass spectrometry and gel based proteomics for identification and quantitation of proteins and their posttranslational modifications (PTM). We have state-of-the-art equipment, including latest additions of Orbitrap Velos (two instruments)and 5800 MALDI TOF/TOF. Our workflows for relative protein quantitation are based on DIGE, label-free, iTRAQ/TMT and SILAC approaches. We can also help investigators identify and quantify protein posttranslational modifications, including phosphorylation, nitrosylation, acetylation, ubiquitination, etc. We provide training in proper sample preparation and lead the researchers through mass spectrometric analysis to data searching and interpretation. Users have access to a variety of proteomics software platforms (Sequest, Mascot, Proteome Discoverer, Scaffold, Protein Pilot) for re-searching the data or viewing the results. In addition to helping the NHLBI investigators, we develop new approaches for PTM characterization and absolute protein quantitation.

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