Genome-wide RNAi screening at the NIH through the Trans-NIH RNAi Initiative

Thursday, October 27, 2011 — Core Poster Session
10:00 a.m. – Noon	South Lobby of Building 10	NHGRI	CORE-17

Authors

• S Martin
• R Guha
• P Tuzmen
• Y Chen
• C Klumpp
• N Caplen
• C Austin

Abstract

Gene silencing through RNAi has emerged as a powerful tool for understanding gene function. Over the past several years, high-throughput RNAi screens have illuminated a wide variety of biology ranging from genes that affect the activity of therapeutic agents to elucidating novel components of signaling pathways. The NIH has recently established a state-of-the-art RNAi screening facility that is now fully operational and soliciting proposals from any intramural researcher. This facility assists with all stages of projects beginning with assay development through genome-wide siRNA screens, informatics/pathway analysis, and rigorous follow-up. Genome-wide siRNA screens for both human and mouse are available. miRNA mimic and inhibitor libraries are also routinely included in screens. A number of large-scale screens have already been completed in collaboration with investigators from throughout the NIH. These screens have identified novel biology and improved our approach towards the successful application of this powerful technology.

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