The DNA Sequencing and Digital Gene Expression Core

Thursday, October 27, 2011 — Core Poster Session
10:00 a.m. – Noon	South Lobby of Building 10	NCI	CORE-10

Authors

• K Hartman
• P Johnson
• S Shema
• S Thorgeirsson

Abstract

The Core offers digital gene expression using the NanoString’s nCounter Analysis System. This system is a fully automated platform for the next generation of digital gene expression analysis. This unique technology is completely non-enzymatic and utilizes fluorescent barcodes hybridized to individual mRNA molecules to directly count the relative abundance of each transcript of interest in a sample. The assay is performed directly on total RNA (no RT, IVT, and PCR) or lysates, from a wide variety of sample types including cells, tissues, FFPE, and blood. It enables multiplexed profiling of 20 to 500+ genes. The platform is ideal for miRNA profiling, pathway expression profiling, validation of complex gene expression signature and high throughput RNA-Seq and array validation and CNV analysis.

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