Intestinal permeability in patients with digestive disorders

Wednesday, October 26, 2011 — Poster Session III
10:00 a.m. – Noon	Natcher Conference Center	NINR	CLIN/CULT/AGING/DISPREV-4

Authors

• A Del Valle-Pinero
• H Van Deventer
• A Martino
• N Patel
• A Remaley
• W Henderson

Abstract

Up to 30% of U.S. individuals suffer from digestive disorders, which are associated with abnormal intestinal permeability (IP). The aim of this study was to calculate IP in patients with digestive disorders compared to controls by measuring urine sugar concentrations prospectively via mass spectrometry after the ingestion of IP test solution. Fasting participants drank IP test solution (100 ml: sucralose, sucrose, mannitol, and lactulose). Urine excreted over 5 hours was collected and kept frozen at -80°C. Urine sugar concentrations were measured using an Agilent 1200 HPLC coupled to an Agilent 6460 Triple quadruple mass spectrometry equipped with an electrospray ionization source using Agilent Jet Stream Technology. 100 μL of methanol containing raffinose as internal standard was added to 100 μL of urine then 20 μL were injected into a ZORBAX Carbohydrate Analysis Column. The sugars were separated with an isocratic water methanol gradient and introduced into the mass spectrometer operated in negative ionization multiple reaction monitoring mode (MRM transitions: 505.2/282.1, 505.2/147raffinose; 395/359sucralose; 341.1/179, 341.1/119sucrose; 181.1/119, 181.1/101.1mannitol and 341.1/161.1lactulose). Altered intestinal permeability was seen in patients with digestive disorders compared to controls. We report a clinically meaningful and noninvasive assessment method for IP alterations in patients with digestive disorders.

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