Macrophage polarization in Ccl2/Cx3cr1 double deficient mice

Wednesday, October 26, 2011 — Poster Session III
10:00 a.m. – Noon	Natcher Conference Center	NEI	CLIN/CULT/AGING/DISPREV-23

Authors

• D Shen
• Y Wang
• J Tuo
• C Chan

Abstract

Purpose: To investigate the polarization of M1 and M2 macrophage associated chemokines, CXCL11, CCL22, and IL-17 at different ages in wild type (WT) and Ccl2/Cx3cr1double deficient (DKO) mice, a retinal degenerative model (AMD). Methods: Eyes of WT and DKO mice at age 20 days, 2, 4, 7 and 12 months were examined by funduscopy. They were then harvested for CXCL11, CCL22 and IL-17 measurement by quantitative RT-PCR. The mouse universal RNA was used as a reference. Results: Funduscopy showed multiple small retinal lesions in 20 day old DKO mice. The lesions became larger, more confluent, and/or atrophic with aging. RT-PCR results show the transcripts of CXCL11, CCL22 and IL-17 in eyes increased with age in both WT and DKO. Furthermore, CXCL11 and IL-17 was significantly higher in DKO than in WT, p value between the two strains are less than 0.05 at all age, while CCL22 was lower in DKO than in WT at all ages, but a significant difference was only found at age 20 day and 2 month. Conclusion: M1 macrophage polarization and IL-17 elevation was observed in aging mice, especially in DKO mice. These mechanisms may play a role in AMD-like lesions in DKO.

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