Derivation of Neural Stem Cells from Human Adult Peripheral CD34+ Cells for Neuroinflammation Study

Friday, November 08, 2013 — Poster Session III
10:00 a.m. – 12:00 p.m.	FAES Academic Center (Upper-Level Terrace)	NINDS	STEMCELL-14

Authors

T. Wang
E. Choi
M.C. Monaco
E. Campanac
M. Medynets
T. Do
P. Rao
K.R. Johnson
A.G. Elkahloun
G. Von Geldern
T. Johnson
S. Subramaniam
D. Hoffman
E. Major
A. Nath

Abstract

Activated T cells inhibit neurogenesis in adult animal brains and cultured human fetal neural stem cells (NSC). However, the role of inhibition of neurogenesis in human neuroinflammatory diseases is still uncertain because of the difficulty in obtaining adult NSC from patients. Recent developments in cell reprogramming suggest that NSC may be derived directly from adult fibroblasts. To develop a method to generate NSC human peripheral CD34+ cells by direct conversion and evaluate whether it can be used for neuroinflammation study, CD34+ cells purified from adult peripheral blood were transfected with Sendai virus containing transcription factors Sox-2, Oct3/4, C-MyC and Klf-4, followed by culturing in NSC selective media. The derived NSC were nestin and SOX-2 positive and OCT4 negative. The cells were differentiated to functional neurons and glial cells by induction using corresponding differentiation media. Activated T cells inhibited NSC proliferation and neuronal differentiation but enhanced oligodendrocyte progenitor proliferation. These results indicate that NSC can be generated from adult CD34+ cells in a relatively short period of time by direct conversion. We have established an unique in vitro model to study the pathophysiology of neuroinflammatory diseases.