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Characterization of responses of human embryonic stem cells to low, clinical diagnostic relevant, doses of ionizing radiation

Friday, November 08, 2013 — Poster Session III

10:00 a.m. – 12:00 p.m.

FAES Academic Center (Upper-Level Terrace)




  • M.V. Sokolov
  • V. Nguyen
  • R.D. Neumann


Even though multiple reports exist in the scientific literature regarding human responses to ionizing radiation (IR), the underlying molecular mechanisms and pathways are still far from being fully understood, partly due to the lack of suitable model systems. We established a novel, human embryonic stem cell (hESC) culture – based model to examine the radiobiological effects in humans. There is a great deal of uncertainty on how low (less than 0.1 Gy) doses of IR affect human cells, and which signaling networks govern cellular responses to such IR exposures. The apparent scarcity of such data is in a marked contrast to practical societal needs to predict health risks emerging from diagnostic radiation, natural background radiation, and environmental radiological accidents. In our present study, we aimed to elucidate the gene expression changes in a panel of hESCs following 0.01 Gy, 0.05 Gy, 0.1 Gy, and, as a reference, 1 Gy of IR. We utilize systems biology approaches, such as DNA microarrays, to interrogate whole genome gene expression alterations; and, we examine the dynamics of transcriptional changes in well-known radiation-responsive set of genes, such as CDKN1A and GADD45A, in more focused studies. The results of our analysis will be presented and discussed.

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