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Cutting Edge Microscopy in the NHLBI Light Microscopy Facility

Thursday, November 07, 2013 — Poster Session II

12:00 p.m. – 2:00 p.m.

FAES Academic Center (Upper-Level Terrace)




  • CA Combs
  • DA Malide
  • X Wu


The NHLBI light microscopy facility is a state-of-the-art microscopy lab that has been helping users of the NHLBI_DIR since 2000. The mission of this facility is to provide state of the art equipment, training, experimental design, and image processing capabilities to assist researchers within the NHLBI-DIR and, in some cases collaborate with other NIH investigators,in experiments involving light microscopy. We have twenty microscopes in five locations. These microscopes provide a wide array of techniques including wide-field fluorescence and transmitted light imaging, confocal, two-photon, total internal reflection fluorescence microscopy (TIRF) and super-resolution microscopy. Drs. Malide, Wu and Combs are experienced microscopists and help users of the facility with all facets of imaging experiments including consultation, training, image analysis, and help with data acquisition. In addition LMF personnel are involved with thier own experiments involving microscopy projects which push the boundries of modern microscopy techniques including enhanced two-photon microscopy and collaborations with researchers improving super-resolution imaging. We also have many image processing techniques available including quantification of image intensities, morphological analysis, co-localization analysis, particle tracking, 3D volume reconstruction, etc. This poster will highlight some of our innovative new microscopy techniques and the various capabilities provided by the facility.

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