October 9 - 12, 2012
Building 10 & Natcher Conference Center
- General Schedule of Events
- Opening Plenary Session
- Concurrent Symposia Sessions
- Poster Sessions
- National Graduate Student Research Conference
- FARE Award Ceremony
- Special Exhibits on Resources for Intramural Research
- TSA Research Festival Exhibit Show
- Clinical Center Tours
- Research Festival Committees
- Past Research Festivals
Trans-NIH Electron Microscopy Shared Facility
| Thursday, November 07, 2013 — Poster Session II | |||
|---|---|---|---|
12:00 p.m. – 2:00 p.m. |
FAES Academic Center (Upper-Level Terrace) |
NIBIB |
RSCHSUPP-40 |
Authors
- G. Zhang
- V. Speransky
Abstract
The Trans-NIH Electron Microscopy Facility situated in NIBIB’s Biomedical Engineering and Physical Science Shared Resource provides intramural investigators with tools for characterizing subcellular structure using both transmission electron microscopy (TEM) and scanning electron microscopy (SEM) and associated specimen preparation methods. Available techniques include conventional chemical fixation, embedding and sectioning of cells and tissues for TEM, high-pressure freezing and freeze-substitution for optimized specimen preservation, and immunolabeling methods to identify specific proteins based on pre- and post-embedding procedures .The facility provides the capability for determining three-dimensional subcellular structure using automated electron tomography. In addition, investigators have access to methods for determining structure of isolated macromolecular complexes by negative staining, as well as mass mapping based on scanning transmission electron microscopy (STEM) of unstained preparations. The facility includes one of the few SEMs on the NIH campus, providing a capability for determining the surface morphology of cells and biomaterials. Training is offered for some of the instruments in the facility so that researchers can record their own electron micrographs.
