October 9 - 12, 2012
Building 10 & Natcher Conference Center
- General Schedule of Events
- Opening Plenary Session
- Concurrent Symposia Sessions
- Poster Sessions
- National Graduate Student Research Conference
- FARE Award Ceremony
- Special Exhibits on Resources for Intramural Research
- TSA Research Festival Exhibit Show
- Clinical Center Tours
- Research Festival Committees
- Past Research Festivals
Automated, High-Throughput Imaging Systems in NIAMS Light Imaging Section Enhance Data Collection for Now and for the Future.
| Thursday, November 07, 2013 — Poster Session II | |||
|---|---|---|---|
12:00 p.m. – 2:00 p.m. |
FAES Academic Center (Upper-Level Terrace) |
NIAMS |
RSCHSUPP-39 |
Authors
- K.J.M Zaal
- A. Milgroom
- E. Ralston
Abstract
We have recently acquired the Slidescanner SCN400F (Leica) which collects brightfield or fluorescence images from fixed samples on up to 384 glass slides. Equipped with a single lens, it locates and autofocuses histology tissue sections and images them entirely at magnifications up to 40x. Fluorescence scanning of up to five fluorophores is possible but only semi-automated. Scans are sent to a server for viewing by users from their own remote computer. Web-based software allows viewing, screenshots and image exports and quantitative image analysis. Our second automated instrument is the Incucyte Zoom (Essen), an imaging system that fits inside a standard tissue culture incubator. It houses trays that snugly hold a large assortment of tissue culture vessels and one 10 or 20x lens for phase contrast and fluorescence imaging (red & green). Through software, the user chooses the number of fields that will be imaged, how often, and how long. Cultures can be removed for feeds or treatments. The software, also accessible remotely, generates cell growth curves, movies, and fluorescence intensity data on the go. Users appreciate image quality, large automated sampling, and remote access. Storage and IT security issues have brought interesting challenges.
