October 9 - 12, 2012
Building 10 & Natcher Conference Center
- General Schedule of Events
- Opening Plenary Session
- Concurrent Symposia Sessions
- Poster Sessions
- National Graduate Student Research Conference
- FARE Award Ceremony
- Special Exhibits on Resources for Intramural Research
- TSA Research Festival Exhibit Show
- Clinical Center Tours
- Research Festival Committees
- Past Research Festivals
Best Practices for the Stabilization and Isolation of Biomolecules from Fresh Frozen and Fixed Histological Preparations
| Thursday, November 07, 2013 — Poster Session II | |||
|---|---|---|---|
12:00 p.m. – 2:00 p.m. |
FAES Academic Center (Upper-Level Terrace) |
NCI |
RSCHSUPP-35 |
Authors
- A Warner
- Y Golubeva
- B Gouker
- R Smith
- J Matta
- L Sternberg
Abstract
Defining the molecular basis of disease is an essential first step toward developing novel therapeutics. Intrinsic in this are the optimal collection, stabilization, formatting, sampling, and testing of research tissues including patient derived tissues and xenografts. Such preparations may include fresh, frozen, dehydrated, or fixed tissue and cells existing in a wide range of formats including fixed or frozen bulk tissues, formalin fixed paraffin embedded (FFPE) tissue blocks, microtomy ribbons, tissue cores, or cut sections mounted on glass or plastic slides. We present optimized workflows that combine “goal appropriate” tissue harvest and processing with digital pathology as the means to accomplish sample enrichment prior to nucleic acid extraction from these different formats. Subsequent quality and yield assessments indicate significant improvements over prior methods including decreased failure rates during subsequent testing. pathology, histopathology, nucleic acids recovery, target enrichment, laser microdissection.
