Skip to main content
 

Best Practices for the Stabilization and Isolation of Biomolecules from Fresh Frozen and Fixed Histological Preparations

Thursday, November 07, 2013 — Poster Session II

12:00 p.m. – 2:00 p.m.

FAES Academic Center (Upper-Level Terrace)

NCI

RSCHSUPP-35

Authors

  • A Warner
  • Y Golubeva
  • B Gouker
  • R Smith
  • J Matta
  • L Sternberg

Abstract

Defining the molecular basis of disease is an essential first step toward developing novel therapeutics. Intrinsic in this are the optimal collection, stabilization, formatting, sampling, and testing of research tissues including patient derived tissues and xenografts. Such preparations may include fresh, frozen, dehydrated, or fixed tissue and cells existing in a wide range of formats including fixed or frozen bulk tissues, formalin fixed paraffin embedded (FFPE) tissue blocks, microtomy ribbons, tissue cores, or cut sections mounted on glass or plastic slides. We present optimized workflows that combine “goal appropriate” tissue harvest and processing with digital pathology as the means to accomplish sample enrichment prior to nucleic acid extraction from these different formats. Subsequent quality and yield assessments indicate significant improvements over prior methods including decreased failure rates during subsequent testing. pathology, histopathology, nucleic acids recovery, target enrichment, laser microdissection.

back to top