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NHLBI Biophysics Core Facility

Thursday, November 07, 2013 — Poster Session II

12:00 p.m. – 2:00 p.m.

FAES Academic Center (Upper-Level Terrace)




  • G Piszczek


The NHLBI Biophysics Core Facility (BCF) provides state of the art equipment and training to assist investigators in studies of macromolecular interactions, dynamics and stability. The BCF currently has resources to study oligomeric state of biomolecular assemblies, perform measurements of affinity, stoichiometry, kinetics and thermodynamics of interactions between proteins, DNA, RNA and their cognate ligands. Biophysical characterization capabilities include measurements of molecular weight, shape, and conformation of biological macromolecules. Oligomeric state of macromolecular assemblies, including their size and shape, can be studied in the BCF using both analytical ultracentrifugation (AUC) and light scattering techniques (SEC-MALS, DLS). Microcalorimetry is the biophysical method of choice for non-invasive, label-free analysis of biomolecular interactions and stability, measuring the heat effect associated with most physical or chemical processes. The BCF has several Isothermal Titration and Differential Scanning Calorimeters (ITC and DSC) that can be used in those studies. Surface Plasmon Resonance (SPR) is a complimentary method for studying macromolecular interactions that can also provide information on binding kinetics and the BCF is equipped with the Biacore 3000 SPR instrument. Additionally, the BCF users can take advantage of several optical spectroscopy methods, including steady-state and time resolved fluorescence, fluorescence anisotropy and circular Dichroism (CD).

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