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NHLBI Electron Microscopy Core Facility

Thursday, November 07, 2013 — Poster Session II

12:00 p.m. – 2:00 p.m.

FAES Academic Center (Upper-Level Terrace)



* FARE Award Winner


  • MP Daniels
  • PS Connelly
  • CA Brantner


We provide advice, technical services, training and use of facilities to NHLBI intramural scientists when aspects of their research problems require structural resolution below that achievable with the light microscope. Intramural scientists consult with the Core Director and staff to determine the appropriate technical approach. Experiments are scheduled and protocols provided for the steps of sample processing to be done in the scientist's laboratory. The Core provides complete technical services for the completion of the electron microscopy (EM) aspects of most projects. This includes: fixation, embedding, ultra-thin sectioning and transmission EM imaging; computed electron tomography; EM immunocytochemistry; ultra-thin cryosectioning of tissues and cells for immunogold localization of antigens; negative staining of viruses, large proteins and polymers as well as lipid and membrane vesicles; rotary shadowing of large protein molecules and other macromolecules; preparation of platinum replicas of cytoskeletons and “unroofed” cells; scanning EM imaging of small organisms, organs, tissues and cells as well as other materials such as artificial matrices. To facilitate timely completion of projects, we encourage fellows and students to learn and execute standard procedures for the processing of samples as well as to be trained in operation of the electron microscopes to acquire images.

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