October 9 - 12, 2012
Building 10 & Natcher Conference Center
- General Schedule of Events
- Opening Plenary Session
- Concurrent Symposia Sessions
- Poster Sessions
- National Graduate Student Research Conference
- FARE Award Ceremony
- Special Exhibits on Resources for Intramural Research
- TSA Research Festival Exhibit Show
- Clinical Center Tours
- Research Festival Committees
- Past Research Festivals
NHLBI Electron Microscopy Core Facility
| Thursday, November 07, 2013 — Poster Session II | |||
|---|---|---|---|
12:00 p.m. – 2:00 p.m. |
FAES Academic Center (Upper-Level Terrace) |
NHLBI |
RSCHSUPP-10 |
* FARE Award Winner
Authors
- MP Daniels
- PS Connelly
- CA Brantner
Abstract
We provide advice, technical services, training and use of facilities to NHLBI intramural scientists when aspects of their research problems require structural resolution below that achievable with the light microscope. Intramural scientists consult with the Core Director and staff to determine the appropriate technical approach. Experiments are scheduled and protocols provided for the steps of sample processing to be done in the scientist's laboratory. The Core provides complete technical services for the completion of the electron microscopy (EM) aspects of most projects. This includes: fixation, embedding, ultra-thin sectioning and transmission EM imaging; computed electron tomography; EM immunocytochemistry; ultra-thin cryosectioning of tissues and cells for immunogold localization of antigens; negative staining of viruses, large proteins and polymers as well as lipid and membrane vesicles; rotary shadowing of large protein molecules and other macromolecules; preparation of platinum replicas of cytoskeletons and “unroofed” cells; scanning EM imaging of small organisms, organs, tissues and cells as well as other materials such as artificial matrices. To facilitate timely completion of projects, we encourage fellows and students to learn and execute standard procedures for the processing of samples as well as to be trained in operation of the electron microscopes to acquire images.
