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Lipid profile changes in traumatic brain injury using MALDI imaging and electrospray ionization mass spectrometry

Wednesday, November 06, 2013 — Poster Session I

4:00 p.m. – 6:00 p.m.

FAES Academic Center (Upper-Level Terrace)




  • A. Roux
  • K. Baldwin
  • M. Muller
  • S. Jackson
  • D. Barbacci
  • A. Schultz
  • B. Cox
  • A.S. Woods


Traumatic brain injury (TBI) is a major cause of death and disability worldwide. Even identical mechanism of injury causes physical, cognitive, social, emotional, and behavioral effects, and the outcome ranges from complete recovery to permanent disability or death. Further research is needed to investigate the cellular response to mild and moderate traumatic brain injury to identify biomarkers that could be correlated with plasma profile and be used for early clinical diagnosis. Lipids are excellent biomarker candidates. They are the major component of cell membranes, are essential in cell signaling, and are implicated in numerous diseases. We used a rat model to study injury sustained by controlled cortical impact (CCI) to the brain over time (1, 3 and 7 days post trauma). We studied the qualitative and quantitative changes in lipid distribution and localization using imaging mass spectrometry (IMS) and electrospray ionization (ESI) mass spectrometry. Brains were removed and sectioned using a cryostat. Coronal sections were prepared for MALDI imaging and extracted for ESI analysis. Our results demonstrate that changes occur over time after injury and implicated several lipid species possibly involved in the dynamics of membrane disruption and cell signaling, shedding light on the molecular changes over time in TBI.

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