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Chromatin Structural Changes Induced by the Tissue-Specific Eµ Enhancer

Wednesday, November 06, 2013 — Poster Session I

4:00 p.m. – 6:00 p.m.

FAES Academic Center (Upper-Level Terrace)




  • JD Bennett
  • A Ghosh
  • S De
  • K Becker
  • R Sen


Transcriptional enhancers have been proposed to activate transcription independent of their location, distance or orientation with respect to gene promoters. The Eµ intronic enhancer of the mouse immunoglobulin heavy-chain (IgH) locus located on chromosome 12 has been shown to be DNase I hypersensitive and have a significant role in epigenetic modifications, transcriptional regulation, and VDJ recombination. One powerful assay to analyze chromatin structure and identify genomic regulatory elements involved in tissue specific and developmentally regulated gene expression is DNase I hypersensitivity (DHS) coupled with high-throughput sequencing. Whereas the Eµ enhancer is critical for activation of IgH transcription in cis, it remains unknown whether it is also involved in trans regulation of other pro-B cell specific genes. We used genome-wide DHS sequencing to probe the open chromatin structure at the single nucleotide level to determine whether the Eµ enhancer in mouse pro-B cells has trans activity on other transcriptional regulatory elements in the murine genome. Our results show several unique changes in DHS levels between Eµ-/- and control cell-line samples, along with the identification of several novel hypersensitive sites in mouse pro-B cells.

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