October 9 - 12, 2012
Building 10 & Natcher Conference Center
- General Schedule of Events
- Opening Plenary Session
- Concurrent Symposia Sessions
- Poster Sessions
- National Graduate Student Research Conference
- FARE Award Ceremony
- Special Exhibits on Resources for Intramural Research
- TSA Research Festival Exhibit Show
- Clinical Center Tours
- Research Festival Committees
- Past Research Festivals
Distinct Profiles of Vitreous micro RNA in Primary Vitreoretinal Lymphoma and Uveitis
| Wednesday, November 06, 2013 — Poster Session I | |||
|---|---|---|---|
4:00 p.m. – 6:00 p.m. |
FAES Academic Center (Upper-Level Terrace) |
NEI |
MOLBIO-25 |
Authors
- J Tuo
- D Shen
- CC Chan
Abstract
Primary vitreoretinal lymphoma (PVRL) affects the retina, vitreous and optic nerve head. Because of the clinical appearances overlapping, PVRL is often masqueraded as uveitis. This study tests the correlation of miRNA in vitreous with PVRL and uveitis. The study included vitreous specimens from 11 B-cell PVRL and 13 uveitis patients. Six selected samples (PVRL n=3, uveitis n=3) were arrayed for 168 human mature miRNAs. The markers in distinct levels between uveitis and lymphoma were further tested in all samples. The array detected 50 miRs in vitreous samples. The miR-484, miR-197, and miR-132 were higher in the PVRL vitreous, while miR-155, miR-200c, and miR-22* higher in the uveitis vitreous. Groupwise comparison in the larger sample size only shows significant lower miR155 in B-cell PVRL than that in uveitis. Heavy overlap of miR155 levels between B-cell PVRL and uveitis samples was noted. IL-10/IL-6 ratio differentiated B-cell PVRL from uveitis perfectly. The ratios are consistently >1 in B cell PVRL and <1 in uveitis (100% both in sensitivity and specificity). There was reverse correlation between Log IL-10 and miR155 in vitreous samples.
