Skip to main content

Deciphering the expressed antibody V gene repertoire in malaria

Friday, November 08, 2013 — Poster Session IV

2:00 p.m. – 4:00 p.m.

FAES Academic Center (Upper-Level Terrace)




  • S Zinocker
  • JN Schickel
  • ER Meffre
  • SK Pierce


Individuals living in malaria endemic areas gradually acquire conventional parasite-specific memory B cells (MBC) as well as a large population of atypical MBCs that are associated with chronic infectious diseases, including AIDS. At present, we know little about the molecular mechanisms underlying the generation of conventional and atypical MBCs in response to malaria. To gain insight into these processes we are sequencing the variable (V) segments of the heavy (H) and light (L) chain genes from hundreds of individual conventional and atypical MBCs from the peripheral blood of children and adults living in a malaria endemic area of Mali. The analyses of these paired VH and VL sequences will allow us to determine the germline VH and VL gene usage in the conventional and atypical MBC population and the relationship between the two. In addition, the number and nature of somatic hypermutations in the VH and VL genes will provide insights to the role of antigen-driven selection in the development of conventional and atypical MBCs. By this analysis we aim to gain a better understanding of the generation of both conventional and atypical MBC during the acquisition of antibody-dependent immunity in malaria.

back to top