October 9 - 12, 2012
Building 10 & Natcher Conference Center
- General Schedule of Events
- Opening Plenary Session
- Concurrent Symposia Sessions
- Poster Sessions
- National Graduate Student Research Conference
- FARE Award Ceremony
- Special Exhibits on Resources for Intramural Research
- TSA Research Festival Exhibit Show
- Clinical Center Tours
- Research Festival Committees
- Past Research Festivals
Comparative analysis of CD8 T cell response to cytomegalovirus or influenza virus in healthy human adults
| Friday, November 08, 2013 — Poster Session IV | |||
|---|---|---|---|
2:00 p.m. – 4:00 p.m. |
FAES Academic Center (Upper-Level Terrace) |
NIA |
IMMUNO-3 |
Authors
- G. Chen
- A. Solokina
- M. Oelke
- K. Madara
- R. Wersto
- J. Schneck
- N.P. Weng
Abstract
The competency of antigen-specific lymphocytes plays critical roles in the adaptive immune response. However, little is known about the differences in the competency of antigen-specific T cells against different viruses. Here, we compared the CD8 T cell responses of 23 healthy adults to CMV-pp65 or Flu-M1 peptides using artificial-antigen-presenting- system in vitro. We found that CMV+ or Flu+ CD8 T cell responses varied in both proliferation and Granzyme-B production and were independent of the frequency of initial antigen-specific CD8 T cells. As expected, Tcm exhibited better expansion than Tn for both CMV and Flu. Intriguingly, the variations of the expansion between CMV+ and Flu+ CD8 T cells were observed only in Tcm but not Tn cells, indicating the importance of antigen experience. Furthermore, the expansion ability was correlated with the telomere length in initial CD8 T cells positively and expanded antigen-specific CD8 T cells negatively, suggesting roles of telomere length in immune response. Taken together, this study showed that incompetency of antigen-specific CD8 T cells is associated with antigen experience and short telomere length. Currently, we are analyzing the usage of TCR variable genes by a single-cell-RT-PCR-Sequencing method to gain the structure difference of TCR against CMV and Flu antigens.
