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Comparative analysis of CD8 T cell response to cytomegalovirus or influenza virus in healthy human adults

Friday, November 08, 2013 — Poster Session IV

2:00 p.m. – 4:00 p.m.

FAES Academic Center (Upper-Level Terrace)




  • G. Chen
  • A. Solokina
  • M. Oelke
  • K. Madara
  • R. Wersto
  • J. Schneck
  • N.P. Weng


The competency of antigen-specific lymphocytes plays critical roles in the adaptive immune response. However, little is known about the differences in the competency of antigen-specific T cells against different viruses. Here, we compared the CD8 T cell responses of 23 healthy adults to CMV-pp65 or Flu-M1 peptides using artificial-antigen-presenting- system in vitro. We found that CMV+ or Flu+ CD8 T cell responses varied in both proliferation and Granzyme-B production and were independent of the frequency of initial antigen-specific CD8 T cells. As expected, Tcm exhibited better expansion than Tn for both CMV and Flu. Intriguingly, the variations of the expansion between CMV+ and Flu+ CD8 T cells were observed only in Tcm but not Tn cells, indicating the importance of antigen experience. Furthermore, the expansion ability was correlated with the telomere length in initial CD8 T cells positively and expanded antigen-specific CD8 T cells negatively, suggesting roles of telomere length in immune response. Taken together, this study showed that incompetency of antigen-specific CD8 T cells is associated with antigen experience and short telomere length. Currently, we are analyzing the usage of TCR variable genes by a single-cell-RT-PCR-Sequencing method to gain the structure difference of TCR against CMV and Flu antigens.

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